Deletions and rearrangements of PAX5 gene in B-lineage acute lymphoblastic leukemia
ZHENG Ji-fu, DONG Sha-sha, WANG Qian, PAN Jin-lan, CHEN Su-ning, QIU Hui-ying
Published 2013-09-10
Cite as Chin J Med Genet, 2013,30(05): 549-552. DOI: 10.3760/cma.j.issn.1003-9406.2013.05.009
Abstract
Objective To determine the frequency paired-box domain 5 (PAX5)gene alterations in Blineage acute lymphoblastic leukemia (B-ALL) harboring 9p abnormalities and its implication for clinical prognosis.Methods Bacterial artificial chromosomes RP11-344B23 and RP11-652D9 encompassing the PAX5 gene were selected.DNA was extracted with conventional method and labeled with fluorescein by nicking transition.Fluorescence in situ hybridization (FISH) was used to determine the rearrangement or deletion of the PAX5 gene in B-ALL harboring chromosome 9p abnormalities.Clinical and laboratory features of patients were analyzed.Results Fifty cases were analyzed with FISH.Complete deletion was observed in 23 patients (46％),partial deletion was observed in 2 patients (4％),and rearrangement was detected only in 1 case.The total frequency of abnormalities was 52％ (26/50).No significant difference was found in clinical features of patients with or without PAX5 rearrangement or deletion.Conclusion The frequency of PAX5 gene alterations in B-ALL harboring 9p abnormalities was 52 ％.However,no significant difference was found between patients with and without PAX5 alterations.
Key words:
B-lineage acute lymphoblastic leukemia; PAX5 gene; 9p abnormalities
Contributor Information
ZHENG Ji-fu
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Suzhou, Jiangsu 215006, P.R.China
DONG Sha-sha
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Suzhou, Jiangsu 215006, P.R.China
WANG Qian
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Suzhou, Jiangsu 215006, P.R.China
PAN Jin-lan
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Suzhou, Jiangsu 215006, P.R.China
CHEN Su-ning
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Suzhou, Jiangsu 215006, P.R.China
QIU Hui-ying
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of the Ministry of Health, Suzhou, Jiangsu 215006, P.R.China
